Project Title:Multi nano-nutrients: preparation and foliar application in agriculture Year:2016 Funding agency: ICAR-NRM Principal Investigator: Dr.Govindaraju Collaboarator: Dr.K.S.Subramanian, TNAU, Coimbatore
Micronutrients (MN) are needed for plants in micro quantities which help in the routine metabolism of plants. Micronutrients are critical in the defense against crop diseases and they commonly result in production of primary and secondary metabolites which are formed as a result of a cascade of reactions. It even confers nutrition based promotion of host resistance. They play a significant role in the crop cultivation and agriculture. Soil is the main source of micronutrients for plants and the availability and deficiency of the micronutrients depends on many soil and environmental factors. The present work focuses on the preparation of nano m icronutrients (iron and zinc) and their foliar application of agriculture.
Project Title:Studies on the impact of elevated levels of water temperature and acidity on the developmental stages, biochemical, immunological and molecular responses in marine organisms (artemia, rotifer and Penaeus vannamei) as model Year:2016 Funding agency: ICAR-NICRA Principal Investigator: Dr.Govindaraju Co-PI: Dr.Radhika Rajasree, Dr.D.Inbakandan, Dr.L.Stanley Abraham, Dr.V.Ganesh Kumar
Relevant research is required for aquaculture to adapt to climate change and countries and regions need to streamline work on issues such as new diseases and preventive treatments, aquatic animal physiology, the search for new and better adapted species, better feeds and feeding practices that are more ecosystem friendly.Ocean acidification, salinity and increasing temperature are the key environmental variables. The present project to study the impact of temperature, salinity and acidity on the developmental stages, biochemical, immunological parameters and molecular stress gene expression studies using artemia, rotifer and shrimp Penaeus vannamei as model is proposed.
Project Title:Awareness, Survey and Conservation of Rays in Indian Coast Lines Year:2016-2017 Funding agency: The Rufford Foundation, United Kingdom (19485-1) Principal Investigator:Mr. C. Kumar Mentor: Dr. D. Inbakandan
Sharks and Rays are the major capture fishery resources of Indian EEZ and are being continuously exploited. In India, the production of elasmobranchs in 2014 was estimated at 47242 tons. According to Wildlife Protection Act, 1972, Government of India has protected the shark and ray species. Recently, Under the Convention on International Trade in Endangered Species of Wild Fauna and Flora, India has listed five shark species and two species of manta rays for conservation. However, landings of the protected species have still been recorded from the Indian coast line. As rays are especially vulnerable to overfishing this work will be created awareness among fisherman, boat owners, NGO volunteers, fisheries extension officials and policy makers, etc. The present work will contribute to reducing fishing pressure on protecting rays, support species specific conservation management plans.
Project Title:Medicinal plants mediated green synthesis of gold nanoparticles and its antimalarial activity against Plasmodium falciparum and Plasmodium berghei Year:2016 Funding agency: National Post doctoral Fellowship (DST-SERB-NPDF) Principal Investigator: Dr. Jayaseelan C Mentor: Dr. Radhika Rajasree S.R.
About half of the world’s population is exposed to the risk of malaria. Malaria poses a major challenge to scientists working on drug discovery and delivery due to their intracellular and strewn locations. The objective of the present study is to develop safe and affordable new drug against malarial parasites that are resistant to existing drugs. Over a few years, nanoparticles used in the field of nanomedicine and has altered the foundations of disease diagnosis, treatment and prevention based on their properties. In this context, the present project is proposed to determine the antimalarial efficacy of eco-friendly green synthesized gold nanoparticles using the medicinal plant extracts against Plasmodium falciparum and Plasmodium berghei.
Project Title:Isolation of active molecules from sponge associated microbes and development of marine natural product-based antifouling coatings Year:2016-2018 Funding agency: DST-SERB National Post doctoral fellowship (PDF/2016/000989) Principal Investigator:Dr. S. Nalini Mentor: Dr. D. Inbakandan
Marine sponges and its associated microbes are known for its promising active metabolites or natural compounds. The present study will screen and identify new natural compounds to combat marine antifouling. Aim of the study is to develop natural coating with durability, cost effectiveness and non-toxic to marine ecosystem. Potent microbes will be isolated from sponge resources and screened for bioactivity. Marine bacterial adhesion, algal adhesion and larval adhesion will be tested in the identified extract. Promising elutes will be taken for compound purification and identification. The purified natural compound will be coated on different surfaces and evaluated in open sea condition for the antifouling potential. The identified natural antifouling coating will be patented and taken for industrial application.
Project Title:Studies on Adsorption characteristics of Radionuclides by Phyco-colloids of Seaweeds Year:2015-2017 Funding agency: AERB, Mumbai, India Principal Investigator: Dr. L. Stanley Abraham
Co-Investigators: Dr. S. R. Radhika Rajashree, Dr. D. Inbakandan & Dr. K. Govindaraju.
Project Co-Ordinators: Dr. V. P. Venugopalan, Dr. Diptendu Das & Dr. Jayashree Ramkumar
Radioactive waste is an inevitable residue from the use of radioactive materials in industry and medicine sector as well as emergency situations during nuclear power plant accidents such as Chernobyl and Fukushima disaster, which in turn has a harmful effect on all living organisms. The management and disposal of such waste is therefore, an issue relevant to almost all countries. In the aspect of radiological safety Cesium, Cobalt and Strontium are major radioactive isotopes because of their relatively long half-lives or high concentrations in the liquid wastes. The development of new materials for removal of radionuclides is the key issue and has significant attention. Application of phyco-colloids from seaweeds for the sorption/desorption of heavy metal and radionuclides in the environment has gained more attention due to its structural interaction and physio-chemical properties. In this AERB funded the objective is to develop natural decorporating agents and recover the radionuclides from contaminated sites by screening and optimizing the bioadsorption or chelation of selected radionuclides Thorium, Strontium and Cesium at different concentrations by phyco-colloids of seaweed biomass.
Project Title:Isolation, Culture and Characterization of Fish Embryonic Stem Cells and Their Application in Transgenesis Year:2015-2018 Funding agency: DST-SERB Principal Investigator:Dr. V. Parameswaran
Embryonic (ES) cell lines are undifferentiated long-term cell cultures derived from inner cell mass of developing embryos. These cells retain ES cell characteristics and can be induced to differentiate into a variety of cell types. When introduced into host embryo, the ES cells can participate in normal development and contribute to several tissues of the host including cells of the germ line. In this project, we propose to develop and characterize ES cells from an established fish model, Danio rerio. Further we will confirm that established ES cells shows all the in vitro properties similar to those observed for rodent and human ES cells. In addition, we will demonstrate in vivo germ line competence of established ES cell lines.
Project Title: Studies on ecotoxicology of engineered nanoparticles in selected marine organisms Year:2007 Funding agency: Department of Biotechnology Principal Investigator: Dr.Radhika Rajasree S.R. Co-Investigators: Dr.V.Ganesh Kumar, Dr.L.Stanley Abraham, Dr.R.Venkatesan
The present project gave the information on varied biological effects of nanoparticle forms on marine organisms and assessed the toxicity of water-soluble fullerene and TiO2 in selected marine organisms & also quantified the growth response of Artemia salina, Crassostrea madrasensis and Lates calcarifer in presence of nanoparticles. Oxyradical-induced lipid and protein damage and impact on total glutathione (GSH) levels, in Asian sea bass Lates calcarifer exposed to nanoparticles were also carried out.
Project Title: Microbial ensilation and co-fermentation of shell wastes for recovery of the biopolymer Year:2008 Funding agency: DST-Technology System Development Programme Principal Investigator: L. Stanley Abraham Co-Investigator: Dr. Radhika Rajasree S.R & Dr. Dr.R.Kirubagaran,OSTI,NIOT
An eco-friendly method to isolate and identify protease and deacetylase producing strains from onshore and offshore sediments for effective recovery of chitin and chitosan from shell wastes. The evaluated the bacterial enzyme potential and devloped a co-fermentation methodology for deproteinization and demineralization of shell wastes to recover chitin.
Project Title:Biologically synthesized gold nanoparticles using seaweed Sargassum spp for antidiabetic studies Year:2009 Funding agency: DST -Nanomission Principal Investigator: Dr.V.Ganesh Kumar Co-Investigator: Dr.A.Rajeswari
Seven different Sargassum spp. were collected along the east coast of Tamilnadu and the synthesis and characterization of gold nanoparticles were performed. In vivo antidiabetic study was carried out using Sargassum spp. mediated gold nanoparticles and insulin loaded Sargassum spp. mediated gold nanoparticles. The biochemical and histopathological results suggest the protective effect of the biobased gold nanoparticles. In addition, identification of capping agent in the biosynthesis of gold nanoparticles has been assessed. The gold nanoparticles have anti-inflammatory effect which was evaluated using circulating inflammatory marker and inflammatory markers.
Project Title:Studies on the effect of PFBR on biological life before and after commissioning Year:2011 Funding agency: BHAVINI Principal Investigator:D. Inbakandan & V. Ganesh Kumar Co-PI: L. Stanley Abraham & Radhika Rajsree S.R.
Discharge from Power plants to coastal waters can have a significant impact on Kalpakkam local ecological structure because of its temperature difference in coastal water quality parameters. Proper documentation of coastal water resources requires in a qualitative way for significant inputs to understand the thermal discharge. In the scenario of expanding electricity generation in our country, we need to be vigilant and generate scientific basis for the regulations necessary with regard to thermal discharges for the conditions prevailing in India. Natural water bodies are used as heat sinks especially by power plants. This causes Thermal contamination and need to be regulated and continually monitored to identify its impact on the sedentary organisms, marine sessile invertebrates and other factors determining the marine productivity. The present work is intended to make a realistic assessment on this in the vicinity of the existing and/or forthcoming nuclear power plants, which in-turn generate the baseline information about the persisting environment. Thus this project accumulate data on thermal plume discharge at the kalpakkam coastal waters using hydrology parameters like physical, chemical and biological water quality data and analyze it seasonally and statistically correlated to know the significance for future studies.
Project Title:Monitoring of bioavailability, toxicity and trophic transfer of manufactured metal oxide nanoparticles in aquatic ecosystem Year:2011 Funding agency: IFS Principal Investigator:Dr.Radhika Rajasree
Quantification of growth responses in the marine algae Chaetoceros sps. and copepod P.parvus to various levels of nanoparticle concentrations were done.The study also assessed the trophic transfer of selected nanoparticles through various levels of the food chain as represented by the test organisms selected.
Project Title:Biofabrication of gold nanoparticles as antibreast cancer drug Year:2012 Funding agency: DST-SERB Principal Investigator:Dr.K.Govindaraju
Cancer is one of the leading causes of death worldwide in both men and women. Among the cancers, breast cancer is one of the major issues in health care in terms of morbidity, mortality and therapy costs. Of several prescription drugs in use for cancer treatment, almost 75% are derived from plant species. Medicinal plants have been considered as of great interest, since they could provide health security to people in primary health care. On this basis,the project aims to generate gold nanoparticles having anticancer property using medicinally significant plants and plant compounds.
Project Title:Stabilization and utilization of trash fish and fish processing waste as a slow release nitrogenous organic fertilizer for increased production in vegetable farming Year:2012 Funding agency: DST-WOSB Principal Investigator:Dr.Radhika Rajasree
Marine fish waste discarded from urban regions cause a serious impact in environment and hence proper recycling method is required for effective management of marine waste. The project is undertaken to find suitable disposable method to convert discarded waste into useful organic nitrogen fertilizer for vegetable farming. The study involves finding suitable methods to produce solid organic fertilizer for soil amendment and liquid fertilizer for foliar application. The research work further involves testing the organic fish fertilizer in agricultural fields to analyze the efficacy of the input in growth and yield of vegetable crops and to promote the product among farming society.
Project Title:Technology Business Incubator Year:2012 Funding agency:NSTEDB (DST) Principal Investigator:Dr.B.Sheela Rani, Dr.Radhika Rajasree S.R., Dr.D.Inbakandan, Dr.L.Stanley Abraham & Dr.V.Ganesh Kumar
Sathayabama Institute of Science and Technology Business Incubator (SU-TBI) of Sathyabama Institute of Science and Technology is intend to produce successful entrepreneurship that will eventually make the program financially viable and free-standing, create jobs, commercialize new technologies and strengthen national economies. SU-TBI of Sathyabama will act as a pedestal to help knowledge driven enterprises to establish and prosper under organized scientific guidance. The main objective of SU-TBI is to produce successful entrepreneurship in Marine technology, biotechnology and engineering research. TBI is exclusively for the development and promotion of entrepreneurship in the combined area of marine and bio resource utilization. The objective of establishing TBI at Sathyabama Institute of Science and Technology is on the basis of its accessible potential resources. We are strong in Research and Development and Technology Innovation, up gradation of findings and their subsequent relocation to the respective domains in the thrust area.
Project Title:Utilization of Steel Plant Waste in Manufacture of Paver Blocks and Slag Foams Year:2013 Funding agency:Ministry of Steel Principal Investigator:Dr. V.Ganesh Kumar Co-PI (1): Dr.Bhupeshraja Co-PI (2): Dr.Padmapriya
Steel slag has wide range of chemical and mineral compositions, resulted by different material and smelting conditions. It has the compounds in the form of oxides and silicates. In this research the main objective is to convert steel slag into a porous material, the properties of steel slag and the making of ‘paver blocks’ will be studied. Steel slag will be collected from steel industry. The collected steel slag undergoes crushing, screening and seperation. The separated slag melted in induction melting furnace. In this work slag will be tested as a collision absorbing foam material in automobiles. The moulded paver blocks will be used for exterior flooring.
Project Title:Antibacterial activity against pathogenic Vibrio harveyi and its protective efficacy in penaeid shrimp Year:2014 Funding agency:DST-SERB Principal Investigator:Dr. Ravi Mani
The present study investigates the protection of shrimp Penaeus monodon against V. harveyi using actinomycetes and the modulation of the shrimp non-specific immunity. The antibacterial activity of the shrimp will be determined by treating both in vitro (intramuscular injection) and in vivo (orally with feed) methods at the concentration of 2 mg per animal and 2% of the actinomycetes incorporated with commercially available artificial pellet feed, respectively. The antibacterial activity of actinomycetes will be confirmed by PCR, qPCR, Microbiological methods and bioassay. In this study, antibacterial activity of actinomycetes by in vivo and in vitro methods showed strong antibacterial activity and the immunological parameters. Examination of up and down regulation of immunological parameters in penaeid shrimp after treated with selected potent marine actinomycetes. Further work has to be carried out for the potent actinomycete compound produce in large scale at hatchery level to analyze the efficacy of marine actinomycete.
Project Title:Technology development for Hatchery Production and Evaluation of Probiotic Bacteria as Feed Supplement for Growth, Survival and Colour Enhancement of Marine Angel Fishes Year:2014 Funding agency:Sathyabama Institute of Science and Technology Grants Commission Post-Doctoral Fellow: Dr. M.V.Rajeswari Mentor: Dr.Radhika Rajasree S.R.
The present study has planned to develop successful technology for hatchery production of marine angel fishes for sustainable aquarium trade with 5 different species occurred in Indian waters with the intention of augmenting marine ornamental fishes for sustainable aquarium trade and Brood Stock development,breeding and maintenance of Marine angel fishes. Technology perfection for mass scale production of marine angel fish is also envisaged.
Project Title:Biological evaluation of functionalized gold nanoparticles as potent anti-diabetic agent in regulating glucose transport using in vitro model Year:2014 Funding agency:DAE-BRNS Principal Investigator: Dr.Govindaraju
A worldwide survey reported that the estimated incidence of diabetes and projection for year 2030 is 350 million. The management of diabetes mellitus is considered a global problem and successful treatment is yet to be discovered. However, search for new anti-diabetic drug and to find out their mechanism of action continues. It is in this context that the idea of pharmacologically important biomolecule of plant materials biofunctionalized gold nanoparticles as anti-diabetic nanomaterial by synthesizing with has been conceived. The present proposed work to intervention of nanotechnological application on the control and management of type II diabetes.
Project Title:Land based, closed-cycle culture of indigenous sea urchin species and development of post-harvest protocols for preservation of roes Year:2015 Funding agency: MoES Principal Investigator:Dr.Radhika Rajasree. S.R.
Sea urchins have recently been attracting considerable research interest as sources of a number of highly valuable bioactive compounds that possess antitumour, antiviral, antimicrobial and anticoagulant properties that hold great promise for use in pharmacological applications. Bulk extraction and isolation of these require a resource base that is easily available and dependable. The international scenario presents a picture of overexploitation and habitat degradation wherever the species has been commercially exploited. This underlines the need for viable culture strategies that can ensure a steady supply, both of the edible roes, as well as of other high value products with potential pharmacological uses. The present project envisages to develop a viable land based closed-cycle culture methodology including hatchery technology, applicable on a commercial scale, for indigenous sea urchin species like Tripneustes gratilla.
Project Title:Oral delivery and immunomodulatory effect of quercetin loaded chitosan tripolyphosphate nanoparticles in Vibrio harveyi challenged shrimp, Penaeus monodon and its immune-related gene expressions Year:2014 Funding agency: DST-SERB Principal Investigator:Dr. D.Inbakandan
The study will examine the use of CS/TPP nanoparticles for oral delivery of quercetin in different tissues of shrimp through oral route. The boosting immunomodulatory effect and expression of the fifteen immune-related genes will be also studied in Vibrio harveyi challenged shrimp to confirm the immune boosting effect of quercetin. The CS/TPP nanoparticles will be synthesized and characterized. The results will indicate that quercetin can be easily delivered into shrimp by feeding with CS/TPP nanoparticles and trigger the immunity of shrimp from aquatic pathogens.
Project Title:Status survey and conservation strategy of Elasmobranchs (Sharks) in South east coast of India Year:2015 Funding agency: Rufford Foundation, U.K. Principal Investigator:Mr. T. Marudhupandi Co-Investigators:Dr. Inbakandan
Sharks are overexploited illegally for variety of purposes worldwide. In India, shark resources were greatly affected in the recent years and regrettably information about the stock assessment, status, threatened or endangered species are scanty. In the present study aims initially to document the abundance and diversity of sharks along southeast coast of India, prior to prepare the management plans.
Project Title:Studies on the preparation and efficacy of inactivated viral vaccine against reovirus infection in grass carp. Year:2017-2019 Funding agency: DST-SERB Principal Investigator:Dr. V. Veeramani
Name of the Mentor:Dr. K. Govindaraju
Grass carp reovirus (GCRV) is a pathogen that causes hemorrhagic disease of grass carp. It is the most serious infectious disease of carp and causes serious losses of fingerlings of grass carp. The aim of the study is to prepare viral vaccine for the control of hemorrhagic disease in Grass carp.
Project Title:Anti-cancer efficacy of MgO nanoparticles synthesized using marine alga: In-vitro and In-vivo study for Hepatocellular carcinoma Year:2017 -2019 Funding agency: DST-SERB Principal Investigator:Dr. S. Tamilselvan Mentor:Dr. V. Ganesh Kumar
Cancer is one among the leading causes of death in the world. According to the 2014 World Cancer Report, approximately 14 million new cancer cases and 8.2 million cancer-related deaths were reported in 2012. Current anti-cancer drugs available in market are not target specific and pose several side-effects and complications in clinical management of various forms of cancer, which highlights the urgent need for novel effective and less-toxic therapeutic approaches. Therefore the present work is focused on the preparation of target specific magnesium oxide nanoparticles using seaweed extracts for cancer therapy as a novel alternative to chemotherapy.
Project Title:Production of marine bacterial bioplastic polymer and its nanocomposites for marine antifouling application. Year:2017-2019 Funding agency: DST-SERB Principal Investigator:Dr. G. Kavitha
Name of the Mentor:Dr. D. Inbakandan
Marine biofouling is one of the chief unanswered troubles at present affecting the shipping industries and industrial equipment. Since, biopolymer is a well-known biomaterial that has potential applications in almost all sectors of the economy. The development of reliable, nontoxic and eco-friendly methods in the synthesis of silver nanoparticles Ag-NPs from PHB have been the subject of great interest in Bioprocess technology. The project is aimed at developing commercial-scale technologies for the cultivation, harvesting and processing of marine microbial source, including bacteria, and actinobacteria for biopolymer production. Exploitation of microbial source for PHB production seems highly promising as it has the mutual benefit of producing polymer utilizing its antifouling activity. This work integrates nanotechnology and bacteriology, leading to possible advances in the formulation of new types of antifoulants. Exploitation of these potential silver nanoparticles coated on marine industrial surfaces and study for the impending biofouling control for a new antifouling material into a profitable realism.
Project Title:Assessment of microbiome responsible for the ice-ice disease of the farmed red algae Kappaphycus alvarezii: a case study based on high throughput Illumina sequencing of the V3–V4 region of the 16S rRNA gene. Year:2017 -2019 Funding agency: DST-SERB Principal Investigator:Dr. S. U. Mohammed Riyaz Mentor:Dr. D. Inbakandan
Kappaphycus alvarezii, elkhorn sea moss, is a species of red algae. It is one of the most important commercial sources of carrageenans. Ice-ice disease, the fouling of K. alvarezii is a disease condition of this seaweed, caused by bacteria in the sea water and induces the characteristic "whitening" and hardening of the seaweed's tissues. The present study will be on assessment of microbiome in ice ice disease of K. alvarezii by metagenomic sequencing of 16S rRNA genes of V3-V4 region in all organisms present in a given complex sample. The method enables us to evaluate bacterial diversity and detect the abundance of microbes in various environments it also provides a means to study unculturable microorganisms that are otherwise difficult or impossible to analyze. Hence, the proposed work may uncover the important microbiome involved in pathogenesis of ice-ice disease in K. alvarezii.